We first established a range of key gene circuit components compatible with plant cell functionality. Here we present a suite of functional recombinase-based gene circuits for use in plants. Synthetic gene circuits have the potential to integrate multiple customizable input signals through a processing unit constructed from biological parts to produce a predictable and programmable output. 230000001172 regenerating Effects 0.Plant biotechnology predominantly relies on a restricted set of genetic parts with limited capability to customize spatiotemporal and conditional expression patterns.229940005550 Sodium alginate Drugs 0.000 claims description 7.238000002744 homologous recombination Methods 0.000 claims description 8.108020004707 nucleic acids Proteins 0.000 claims description 10.241001233957 eudicotyledons Species 0.000 claims description 10.238000011069 regeneration method Methods 0.000 claims description 13.239000002157 polynucleotide Substances 0.000 claims description 59.229920000023 polynucleotide Polymers 0.000 claims description 59.150000007523 nucleic acids Chemical group 0.000 claims abstract description 19.
101700006494 nucA Proteins 0.000 claims abstract description 67.238000001943 fluorescence-activated cell sorting Methods 0.000 title claims description 49.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to US201261697890P priority Critical Priority to US61/697,890 priority Priority to US14/020,694 priority Priority to US14/020,694 priority patent/US20140075593A1/en Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc Publication of WO2014039970A1 publication Critical patent/WO2014039970A1/en Publication of WO2014039970A9 publication Critical patent/WO2014039970A9/en Links ( en Inventor German Spangenberg Sareena SAHAB John Mason Original Assignee Dow Agrosciences Llc Priority date (The priority date is an assumption and is not a legal conclusion. Google Patents WO2014039970A1 - Fluorescence activated cell sorting (facs) enrichment to generate plants WO2014039970A1 - Fluorescence activated cell sorting (facs) enrichment to generate plants
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